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Advances in DNA and eDNA-based biomonitoring: Field & lab methods
Edited by DNAqua-Net Working Group 3 leaders: Kat Bruce (UK), Emre Keskin (Turkey) & DNAqua-Net Chair Florian Leese (Germany)

This DNAqua-Net topical issue edited by the leaders of Working Group 3 (Field and lab protocols), Kat Bruce (UK) and Emre Keskin (Turkey), as well as DNAqua-Net Chair Florian Leese (Germany) welcomes submissions on DNA and environmental DNA metabarcoding and metagenomics with a focus on field and lab aspects. Manuscripts can present results on sample collection, preservation of material, comparison of sample volumes or materials used. Laboratory protocol comparisons including benchmarks of different methods, primer comparisons are as welcome as further articles comparing outcomes of different labelling and sequencing strategies. We also welcome submissions that deal with QA/QC in field and lab workflows and method standardisation.

Papers published: 3   |  Total pages: 28
Taxa:
 
Protozoa (1), Fungi (1), Plantae (1), Animalia (2), Invertebrata (1), Chromista (2), Ochrophyta (1)
Regions:
 
DNA and eDNA methods for bioassessment and monitoring: Steps towards formal implementation
Edited by DNAqua-Net Working Group 5 leaders: Pieter Boets (Belgium), Daniel Hering (Germany), Patricia Mergen (Belgium)

This DNAqua-Net topical issue edited by the leaders of Working Group 5 (Implementation and legal challenges) Pieter Boets (Belgium), Daniel Hering (Germany) and Patricia Mergen (Belgium) addresses chances and challenges of DNA based method implementation as part of legal bioassessment and monitoring programs. Defining standards for sampling, DNA analysis and bioinformatic processing of the data is crucial for implementation of DNA based biomonitoring with regard to the European Water Framework Directive, the Marine Strategy Framework Directive and many other international directives such as the US and AU Clean Water Act and many more. For the special issues, we welcome contributions that provide such guidelines for implementation or standardised methods that could help to legally accept the method. We further encourage forum papers that discuss changes and challenges of DNA-based bioassessment for biomonitoring of aquatic ecosystems for various perspectives or review papers that analyse the status quo of method implementation.

Papers published: 2   |  Total pages: 24
Taxa:
 
Animalia (1), Vertebrata (1), Aves (1), Mammalia (1), Heterokonta (1), Pisces (1), Chordata (1)
Regions:
 
Europe (1), Cyprus (1), Germany (1), Central Europe (1)
Metabarcoding, statistical learning and High Performance Computing
Edited by Diego Fontaneto (Italy), Alain Franc (France)

This DNAqua-Net topical issue edited by the leaders of Working Group 4 (bioinformatics), Diego Fontaneto (Italy) and Alain Franc (France) welcomes submissions on DNA and environmental DNA metabarcoding and metagenomics with a focus on bioinformatic processing and infrastructure. Available bioinformatic pipelines cover all the steps from raw sequence data from massive sequencing to the production of publication-standard graphs and statistical analyses. Yet, such pipelines are usually very general and are not devoted to specific needs: here there is still ample space and opportunity to develop tools and approaches to address and solve some of the weaknesses that still exist for specific applications of metabarcoding. The special issues furthermore welcomes submissions that assess and benchmark infrastructure for processing and storing DNA and eDNA data for large scale monitoring.

Papers published: 0
DNA barcode reference libraries for aquatic bioassessment and monitoring
Edited by Fedor Čiampor (Slovakia), Torbjørn Ekrem (Norway), Alexander M. Weigand (Luxembourg)

This DNAqua-Net topical issue edited by the leaders of Working Group 1 (DNA barcode references), Torbjørn Ekrem (Norway), Fedor Ciampor (Slovakia) and DNAqua-Net manager Alexander Weigand (Luxembourg) welcomes submissions on DNA and environmental DNA metabarcoding and metagenomics with a focus on the underlying reference databases. Undoubtly, DNA barcode reference libraries are of immense importance for correct identification of organisms using DNA barcoding and metabarcoding. We welcome contributions that focus on opportunities and challenges related to construction of reference databases and analysis of reference data. Furthermore, we welcome publications that provide and describe reference data for any organismal group.

 

Papers published: 0

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