Primer Validation
Metabarcoding and Metagenomics 7: e103856
https://doi.org/10.3897/mbmg.7.103856 (05 Sep 2023)
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  • ContentsContents
  • Article InfoArticle Info
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  • MetricsMetrics
  • CommentComment
  • RelatedRelated
  • FigsFigs
  • TabsTabs
  • TaxaTaxa
  • DataData
  • RefsRefs
  • CitedCited
  • Article title
  • Abstract
  • Key words
  • Introduction
  • Methods
    • Fish swabs
    • DNA extraction
    • Mock community composition
    • DNA amplification and sequencing
    • Bioinformatics
    • Statistical analyses
  • Results
    • Fish mock community composition
    • DNA extraction, sequencing, and bioinformatics
    • Taxonomic composition comparison
    • Primer bias impact on species detection
    • Evaluation of oversplitting rates
    • PCR replicate consistency assessment
    • Reference database coverage
  • Discussion
    • Detection ability, and reproducibility
    • Read proportions
    • False-negative assignments
    • Ambiguous detections
    • False-positive assignments
  • Conclusion
    • Disclaimer
  • Acknowledgements
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary materials
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