Research Article
Metabarcoding and Metagenomics 7: e98539
https://doi.org/10.3897/mbmg.7.98539 (23 Feb 2023)
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  • ContentsContents
  • Article InfoArticle Info
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  • Article title
  • Abstract
  • Key Words
  • Introduction
  • Methods
    • Creation of a regional library for the Gulf of St. Lawrence (GSL-rl) with a reliability ranking system
    • Evaluating the accuracy and precision of species assignments using the public NCBI-nt repository
    • Contrasting species assignments using the regional library or the public NBCI-nt repository, and popular assignment methods
    • Data accessibility
  • Results
    • A COI regional library with a reliability ranking system for metazoans from the Gulf of St. Lawrence (GSL-rl)
    • Accuracy and precision of species assignments using NCBI-nt and two assignment methods
    • Comparison of the reliability of species assignments to a metabarcoding dataset using GSL-rl and NCBI-nt with three assignment methods
  • Discussion
    • A COI regional library with a reliability ranking system for metazoans from the Gulf of St. Lawrence (GSL-rl)
    • Accuracy of species assignments using NCBI-nt and two assignment methods
    • Comparing the reliability of species assignments to a metabarcoding dataset using GSL-rl and NCBI-nt with three assignment methods
    • Maximizing the reliability and the number of species assignments in eDNA metabarcoding studies by combining the use of a regional library and a public repository
  • Acknowledgements
  • References
  • Supplementary material
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