Research Article
Metabarcoding and Metagenomics 7: e98539
https://doi.org/10.3897/mbmg.7.98539 (23 Feb 2023)
https://doi.org/10.3897/mbmg.7.98539 (23 Feb 2023)
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Article title
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Abstract
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Key Words
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Introduction
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Methods
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Creation of a regional library for the Gulf of St. Lawrence (GSL-rl) with a reliability ranking system
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Evaluating the accuracy and precision of species assignments using the public NCBI-nt repository
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Contrasting species assignments using the regional library or the public NBCI-nt repository, and popular assignment methods
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Data accessibility
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Results
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A COI regional library with a reliability ranking system for metazoans from the Gulf of St. Lawrence (GSL-rl)
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Accuracy and precision of species assignments using NCBI-nt and two assignment methods
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Comparison of the reliability of species assignments to a metabarcoding dataset using GSL-rl and NCBI-nt with three assignment methods
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Discussion
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A COI regional library with a reliability ranking system for metazoans from the Gulf of St. Lawrence (GSL-rl)
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Accuracy of species assignments using NCBI-nt and two assignment methods
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Comparing the reliability of species assignments to a metabarcoding dataset using GSL-rl and NCBI-nt with three assignment methods
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Maximizing the reliability and the number of species assignments in eDNA metabarcoding studies by combining the use of a regional library and a public repository
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Acknowledgements
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References
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Supplementary material
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