Study site

Faecal sample collection and DNA extraction

Molecular identification of samples source

Efficiency of molecular species analysis to assess otter diet

DNA amplification and next-generation sequencing for diet analysis

Sequence analysis and taxon assignment

Data analysis

Traditional survey of fish biodiversity

Relative biomass contribution of prey

Data availability

The vertebrate diet of the Eurasian otter

Seasonal variations in the otter diet

Comparison of the fish composition of otter diets with the results of traditional fish surveys

Relative biomass contribution of prey

Efficiency of species resolution in the evaluation of otter feeding habits

Diet of the Eurasian otter

Efficiency of the molecular analysis of otter feeding habits in surveying environmental fish communities

Species resolution of otter molecular diet analysis

Implications for conservation