Non-destructive insect metabarcoding workflow. Samples are collected by the AVR trap (top left) and Sentinel trap (top right) in a propylene glycol solution (50%) (A); glycol is filtered and samples are examined morphologically and sorted by size, prior to non-destructive DNA extraction (B); partial COI barcode is amplified, Illumina adapters containing unique dual indexes are attached using real-time PCR (C); sample DNA concentrations are then normalised using SequalPrep normalisation plates, the library is pooled and size and concentration are inspected using a TapeStation (D); the final library is sequenced using an Illumina MiSeq and the data are analysed through a bioinformatic pipeline (E). Some details of this figure were created using BioRender (BioRender.com).