A–D. Natural sea water sampling operations at the Pier with a bucket, ca. 3 m (A); at water surface (B) and under water (C) of Moroiso with a 1 L polychlorinated bottle, ca. 2 m; at water bottom of Jogashima, ca. 80 m, 250 m and 270 m with 5L water sampling bottle (D). E, F. Aquarium tanks at Misaki Marine Biological Station, whole view (E) and an individual tank (F).

 
 
  Part of: Okanishi M, Kohtsuka H, Wu Q, Shinji J, Shibata N, Tamada T, Nakano T, Minamoto T (2023) Development of two new sets of PCR primers for eDNA metabarcoding of brittle stars (Echinodermata, Ophiuroidea). Metabarcoding and Metagenomics 7: e94298. https://doi.org/10.3897/mbmg.7.94298