Research Article
Metabarcoding and Metagenomics 7: e86883
https://doi.org/10.3897/mbmg.7.86883 (05 Jul 2023)
Editors' Choice 2023
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  • ContentsContents
  • Article InfoArticle Info
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  • TaxaTaxa
  • DataData
  • RefsRefs
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  • Inst. CodeInst. Code
  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Sampling
    • Bombus identification
    • Pollen metabarcoding laboratory workflow
    • Reference databases and bioinformatic analysis
  • Results
    • Bumblebee specimens and sequencing success
    • Marker choice affects plant taxa detection
    • Pollen metabarcoding as a tool to reveal plant-pollinator interactions
    • Comparison of corbicula and body pollen samples
    • Comparison between historic and recent bumblebee samples
  • Discussion
    • Comparison of recent and historic Bombus samples
    • Plant barcoding markers in pollen metabarcoding
    • Demonstrating the utility of ITS1 in metabarcoding
  • Conclusion
  • Acknowledgements
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Appendix 1
  • Appendix 2
  • Appendix 3
  • Appendix 4
  • Appendix 5
  • Supplementary materials
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