Research Article
Metabarcoding and Metagenomics 7: e86883
https://doi.org/10.3897/mbmg.7.86883 (05 Jul 2023)
https://doi.org/10.3897/mbmg.7.86883 (05 Jul 2023)
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Article title
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Abstract
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Key words
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Introduction
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Materials and methods
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Sampling
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Bombus identification
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Pollen metabarcoding laboratory workflow
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Reference databases and bioinformatic analysis
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Results
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Bumblebee specimens and sequencing success
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Marker choice affects plant taxa detection
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Pollen metabarcoding as a tool to reveal plant-pollinator interactions
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Comparison of corbicula and body pollen samples
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Comparison between historic and recent bumblebee samples
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Discussion
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Comparison of recent and historic Bombus samples
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Plant barcoding markers in pollen metabarcoding
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Demonstrating the utility of ITS1 in metabarcoding
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Conclusion
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Acknowledgements
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Additional information
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Conflict of interest
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Ethical statement
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Funding
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Author contributions
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Author ORCIDs
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Data availability
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References
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Appendix 1
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Appendix 2
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Appendix 3
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Appendix 4
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Appendix 5
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Supplementary materials
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