Research Article
Metabarcoding and Metagenomics 6: e85844
https://doi.org/10.3897/mbmg.6.85844 (24 Nov 2022)
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  • ContentsContents
  • Article InfoArticle Info
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  • TaxaTaxa
  • DataData
  • RefsRefs
  • CitedCited
  • Article title
  • Abstract
  • Key Words
  • Introduction
  • Materials and methods
    • Site selection and sampling
    • Sample preservation methods
    • Storage duration and DNA extraction
    • DNA quality and quantity
    • PCR amplification and sequencing
    • Bioinformatics
    • Statistical analyses
    • Diatom indices calculation
    • Data availability
  • Results
    • DNA quality and quantity
    • Diatom assemblage diversity
    • Diatom assemblage composition
    • Ecological quality index for freshwater sites
  • Discussion
    • Sample preservation: a robust first step in diatom metabarcoding
    • Towards a standardised and user-friendly method
  • Conclusion and perspectives
  • Acknowledgements
  • References
  • Supplementary materials
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