Research Article
Metabarcoding and Metagenomics 6: e85199
https://doi.org/10.3897/mbmg.6.85199 (07 Oct 2022)
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  • Article title
  • Abstract
  • Key Words
  • Introduction
  • Materials and methods
    • Sampling design
    • DNA extraction and Bd qPCRs
    • Amphibian mock community
    • Amphibian DNA metabarcoding-Library preparation
    • Reference database construction
    • Observational data mining
    • Sequence analysis
    • Statistical analyses
  • Results
    • Bd detection and comparison of two TaqMan assays
    • Internal positive control
    • Evaluation of amphibian metabarcoding
    • Amphibian species distribution in Norway
    • Comparison between eDNA and traditional observation monitoring methods
  • Discussion
    • Lessons learned for a national monitoring program based on eDNA
    • Putting our results in the context of previous risk assessment for amphibians due to Bd
  • Acknowledgements
  • References
  • Supplementary materials
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