Kullback-Leibler Divergences between the true community composition and the metabarcoding estimates of it. Community composition is measured in terms of biomass (left) or the number of specimens (right). Data are shown both for the 16S marker (blue) and the COI marker (orange). For the 16S marker, the divergence between the metabarcoding and the original sample increases with buffer aggressiveness and incubation time, while for the COI marker, the divergence is only affected by an increase in buffer aggressiveness.