Concentration and purity of the DNA extracts from different extraction methods. DNA concentration (upper panel) clearly increases with buffer aggressiveness and incubation time using the manual salt saturation purification protocol, while the increase due to incubation time is less clear, but the effect of lysis buffer can still be appreciated when using the robot purification protocol. Note that the starting input volume of lysate is 7.5 mL for the manual purification method and 225 µL (30 times smaller approximately) for the robot, while elution volume is 150 µL in both cases. Purity of the DNA extract (lower panel) is higher for the manual purification and the longer incubation times, regardless of the lysis buffer.