Comparison of the number of species detected between monitoring methods. The number of species detected by eDNA metabarcoding is higher than that of physical surveys (GLMM: p < 0.001). Comparison of community composition at each area was shown via Venn diagrams. Blue and yellow show eDNA metabarcoding and physical surveys, respectively. The number indicates the number of detected species.

 
 
  Part of: Sakata MK, Kawata MU, Kurabayashi A, Kurita T, Nakamura M, Shirako T, Kakehashi R, Nishikawa K, Hossman MY, Nishijima T, Kabamoto J, Miya M, Minamoto T (2022) Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia. Metabarcoding and Metagenomics 6: e76534. https://doi.org/10.3897/mbmg.6.76534