Comparison of amphibian community structures for each area and method. The NMDS plot showed variation of composition. The composition is significantly different between monitoring methods (PERMANOVA: p < 0.001). The ellipses show the 95% confidence level, based on the centroid calculated for each monitoring method.

 
 
  Part of: Sakata MK, Kawata MU, Kurabayashi A, Kurita T, Nakamura M, Shirako T, Kakehashi R, Nishikawa K, Hossman MY, Nishijima T, Kabamoto J, Miya M, Minamoto T (2022) Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia. Metabarcoding and Metagenomics 6: e76534. https://doi.org/10.3897/mbmg.6.76534