Locations of the nine amphibian metabarcoding primer pairs and amplicons on the target mitochondrial 16S rRNA gene. The target gene sequence of Xenopus laevis (Accession ID: PRJNA177353) was used as template. Asterisk shows the primer set evaluated in this study. Note that the amplicon sizes of the primer sets may vary depending on the amphibian species. Others are existing primer sets: Vert-16S (Vences et al. 2016), Ve16S (Evans et al. 2016), L2513/H2714 (Kitano, Umetsu, Tian and Osawa 2007), 16SA-L/16SB-H (Vences, Thomas, Van Der Meijden, Chiari and Vieites 2005).

 
 
  Part of: Sakata MK, Kawata MU, Kurabayashi A, Kurita T, Nakamura M, Shirako T, Kakehashi R, Nishikawa K, Hossman MY, Nishijima T, Kabamoto J, Miya M, Minamoto T (2022) Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia. Metabarcoding and Metagenomics 6: e76534. https://doi.org/10.3897/mbmg.6.76534