Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia

Masayuki K. Sakata; Mone U. Kawata; Atsushi Kurabayashi; Takaki Kurita; Masatoshi Nakamura; Tomoyasu Shirako; Ryosuke Kakehashi; Kanto Nishikawa; Mohamad Yazid Hossman; Takashi Nishijima; Junichi Kabamoto; Masaki Miya; Toshifumi Minamoto

doi:10.3897/mbmg.6.76534

Research Article

Metabarcoding and Metagenomics 6: e76534
https://doi.org/10.3897/mbmg.6.76534 (21 Feb 2022)

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Toshifumi Minamoto (2022)
Environmental DNA analysis for macro-organisms: species distribution and more. DNA Research 29: .

DOI: 10.1093/dnares/dsac018

Ippei Aoshima, Ryohei Nakao, Toshifumi Minamoto, Atushi Ushimaru, Masayuki Sato (2023)
Heterogeneous preference for biodiversity in Japanese urban blue spaces based on people's nature experiences: Analysis using eDNA and satisfaction data. City and Environment Interactions 18: 100101.

DOI: 10.1016/j.cacint.2023.100101

Masaki Takenaka, Koki Yano, Tomoya Suzuki, Koji Tojo (2023)
Development of novel PCR primer sets for DNA barcoding of aquatic insects, and the discovery of some cryptic species. Limnology 24: 121.

DOI: 10.1007/s10201-022-00710-5

Masaki Takenaka, Yuta Hasebe, Koki Yano, Seiya Okamoto, Koji Tojo, Masashi Seki, Shun Sekiguchi, Takeshi Jitsumasa, Nobuyuki Morohashi, Yoshihiro Handa, Tomoka Sakaba (2024)
Environmental DNA metabarcoding on aquatic insects: Comparing the primer sets of MtInsects‐16S based on the mtDNA 16S and general marker based on the mtDNA COI region. Environmental DNA 6: .

DOI: 10.1002/edn3.588

Manuel Alejandro Coba-Males, Pablo Medrano-Vizcaíno, Sandra Enríquez, David Brito-Zapata, Sarah Martin-Solano, Sofía Ocaña-Mayorga, Gabriel Alberto Carrillo-Bilbao, Wilmer Narváez, Jaime Antonio Salas, Jazzmín Arrivillaga-Henríquez, Manuela González-Suárez, Ana Poveda, Ruslan Kalendar (2023)
From roads to biobanks: Roadkill animals as a valuable source of genetic data. PLOS ONE 18: e0290836.

DOI: 10.1371/journal.pone.0290836

Joshua P. Newton, Philip W. Bateman, Matthew J. Heydenrych, Joshua H. Kestel, Kingsley W. Dixon, Kit S. Prendergast, Nicole E. White, Paul Nevill (2023)
Monitoring the birds and the bees: Environmental DNA metabarcoding of flowers detects plant–animal interactions. Environmental DNA 5: 488.

DOI: 10.1002/edn3.399

Dongyi WU, Pingshin LEE, Hongman CHEN, Fang YAN, Jiayue HUANG, Yanhong HE, Ruiyao WU, Zhiyong YUAN (2024)
Validation and development of eDNA metabarcoding primers for comprehensive assessment of Chinese amphibians. Integrative Zoology : .

DOI: 10.1111/1749-4877.12832

Mark S. Johnson, David Pillard (2023)
Amphibian Species in Environmental Risk Assessment Strategies. : 6.

DOI: 10.1039/9781837672004-00006

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