Variation of ASVs and species in number of pooled DNA extracts. The percentage of unique and shared ASVs (a) or species (b) between the separate and pooled DNA extracts are shown for the different locations. These percentages are calculated based on UpSet plots. Therefore, sets with the different replicates were made for the separate DNA extracts (1,2,3,4,5,6) and pooled DNA extracts: two (2a,2b,2c), four (4a,4b,4c) and six (6a,6b,6c). (c) The unique number of species in the separate and pooled DNA extracts (2, 4 or 6) for each location.

 
 
  Part of: Van den Bulcke L, De Backer A, Ampe B, Maes S, Wittoeck J, Waegeman W, Hostens K, Derycke S (2021) Towards harmonization of DNA metabarcoding for monitoring marine macrobenthos: the effect of technical replicates and pooled DNA extractions on species detection. Metabarcoding and Metagenomics 5: e71107. https://doi.org/10.3897/mbmg.5.71107