Variation of ASVs and species in DNA replicates. The percentage of unique and shared ASVs (a) or species (b) between all six DNA replicates are shown for locations with low, medium or high diversity. These percentages were calculated based on UpSet plots of the different DNA replicates in biological replicates.

 
 
  Part of: Van den Bulcke L, De Backer A, Ampe B, Maes S, Wittoeck J, Waegeman W, Hostens K, Derycke S (2021) Towards harmonization of DNA metabarcoding for monitoring marine macrobenthos: the effect of technical replicates and pooled DNA extractions on species detection. Metabarcoding and Metagenomics 5: e71107. https://doi.org/10.3897/mbmg.5.71107