Best fitting number of clusters (k=2) using the unsupervised machine learning k-means for the combined identifications of marine macrozoobenthic species using both COI-primers (COI – red) and the 18S-primer (18S – blue), in the three sampling times (3M – 3 months; 7M – 7 months; 10M – 10 months; 15M – 15 months) and the three artificial substrates (Sla – slate; PVC; Gra – granite).

 
 
  Part of: Leite BR, Vieira PE, Troncoso JS, Costa FO (2021) Comparing species detection success between molecular markers in DNA metabarcoding of coastal macroinvertebrates. Metabarcoding and Metagenomics 5: e70063. https://doi.org/10.3897/mbmg.5.70063