Sequencing primer and two-step PCR layout. Note: The template specific primers used in this example are for demonstration purposes only and vary in each unique PCR setup. Additional template (x) strands bending away from the primer sequence in the 1st PCR round demonstrate their non-complementarity.

 
 
  Part of: Kolter A, Gemeinholzer B (2021) Internal transcribed spacer primer evaluation for vascular plant metabarcoding. Metabarcoding and Metagenomics 5: e68155. https://doi.org/10.3897/mbmg.5.68155