Internal transcribed spacer primer evaluation for vascular plant metabarcoding

Andreas Kolter; Birgit Gemeinholzer

doi:10.3897/mbmg.5.68155

Ancuța Cristina Raclariu-Manolică, Quentin Mauvisseau, Renato Paranaiba, Hugo J. De Boer, Carmen Socaciu (2023)
Authentication of milk thistle commercial products using UHPLC-QTOF-ESI + MS metabolomics and DNA metabarcoding. BMC Complementary Medicine and Therapies 23: .

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Mary Sadyrova, Emily Martin, Philip Ramsey, Lorinda Bullington (2025)
Mock Plant Communities and a Large Mammal Case Study Reveal ITS2 Primer Bias Against Graminoids. Ecology and Evolution 15: .

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Y. O. Tynkevich, T. S. Hrek, I. G. Olshanskyi, I. I. Panchuk, R. A. Volkov (2025)
Genetic polymorphism of the invasive species Impatiens parviflora DC. in Ukraine. Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 22: 10.

DOI: 10.7124/visnyk.utgis.22.1-2.1684

Thomas Reinhart, Armando Espinosa Prieto, Thomas Begoc, Hugues Tinguy, Francis Bick, Etienne Chanez, Jean-Nicolas Beisel, Laurent Hardion (2025)
A comparative analysis of hybridisation capture and PCR-based eDNA metabarcoding for monitoring bryophytes in riparian ecosystems. Metabarcoding and Metagenomics 9: .

DOI: 10.3897/mbmg.9.154548

Josh Boyte, Sarah M. Sherson, Lachlan Tegart, Nithya Fernandopulle, Hee Sung Kang, Edwin R. Lampugnani, Jonathan Pham, Graham A. Mackay, Edward J. Newbigin, John F. Golz (2026)
An integrated approach identifying seasonal variation in allergenic grass pollen in southern Australia. Environmental Research 301: 124518.

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Jasmine J. M. Ong, Max D. Y. Khoo, Li Si Tay, Bryan T. M. Lim, Charles J. Marsh, Melvyn Aik San Tan, Wen Xuan Chiok, G. Kenny Png, Matti A. Niissalo, Gillian S. W. Khew, Marcus A. H. Chua, Malcolm C. K. Soh, Matthew Scott Luskin, Jun Ying Lim (2026)
Land use legacies shape wild boar (Sus scrofa) diet along a forest-urban gradient in Singapore. Urban Ecosystems 29: .

DOI: 10.1007/s11252-026-02032-1

Robert S. Cornman, Mark J. Hepner, Clint R.V. Otto (2026)
The Appalbees menu: a multiyear, multilocus metagenetic assessment of pollen foraging by Appalachian Bombus affinis workers. PeerJ 14: e20284.

DOI: 10.7717/peerj.20284

Y. O. Tynkevich, D. V. Yakobyshen, A. S. Cherkazianova, A. Y. Shelyfist, R. A. Volkov (2024)
Intragenomic Polymorphism of the ITS1-5.8S-ITS2 Region in Invasive Species of the Genus Reynoutria. Cytology and Genetics 58: 536.

DOI: 10.3103/S0095452724060112

Marko Prous, Andrew Liston, Spencer K. Monckton, Katja Kramp, Hege Vårdal, Veli Vikberg, Erik Heibo, Marko Mutanen (2025)
West Palaearctic species of Euura Newman, 1837 (Hymenoptera, Tenthredinidae). European Journal of Taxonomy 977: .

DOI: 10.5852/ejt.2025.977.2799

Isabel C. Kilian, Stephanie J. Swenson, Ximo Mengual, Birgit Gemeinholzer, Andrée Hamm, J. Wolfgang Wägele, Ralph S. Peters (2023)
More complex than you think: Taxonomic and temporal patterns of plant–pollinator networks of caraway (Carum carvi L.). Molecular Ecology 32: 3702.

DOI: 10.1111/mec.16943

Armando Espinosa Prieto, Jean‐Nicolas Beisel, Pieternel Verschuren, Laurent Hardion (2023)
Toward freshwater plant diversity surveys with eDNA barcoding and metabarcoding. Environmental DNA 5: 648.

DOI: 10.1002/edn3.407

Leighton J Thomas, Ameli Kirse, Hanna Raus, Kathrin Langen, Björn Nümann, Georg Tschan, Birgit Gemeinholzer, J. Wolfgang Wägele, Sarah Bourlat (2024)
Synchronised monitoring of plant and insect diversity: a case study using automated Malaise traps and DNA-based methods. Biodiversity Data Journal 12: .

DOI: 10.3897/BDJ.12.e127669

Alexander Edwards, Birgit Gemeinholzer (2025)
Case study of non-lethal sampling for plant-pollinator networks via barcoding and metabarcoding on bumble bees in Germany. Metabarcoding and Metagenomics 9: .

DOI: 10.3897/mbmg.9.141904

Armando Espinosa Prieto, Laurent Hardion, Nicolas Debortoli, Jean‐Nicolas Beisel (2024)
Finding the perfect pairs: A matchmaking of plant markers and primers for multi‐marker eDNA metabarcoding. Molecular Ecology Resources 24: .

DOI: 10.1111/1755-0998.13937

Stephanie J. Swenson, Lisa Eichler, Thomas Hörren, Andreas Kolter, Sebastian Köthe, Gerlind U. C. Lehmann, Gotthard Meinel, Roland Mühlethaler, Martin Sorg, Birgit Gemeinholzer (2022)
The potential of metabarcoding plant components of Malaise trap samples to enhance knowledge of plant-insect interactions. Metabarcoding and Metagenomics 6: .

DOI: 10.3897/mbmg.6.85213

Andreas Kolter, Martin Husemann, Lars Podsiadlowski, Birgit Gemeinholzer (2023)
Pollen metabarcoding of museum specimens and recently collected bumblebees (Bombus) indicates foraging shifts. Metabarcoding and Metagenomics 7: .

DOI: 10.3897/mbmg.7.86883

Andreas Kolter, Paul Hebert (2025)
Automating the Curation of DNA Barcode Databases for Vascular Plants. Environmental DNA 7: .

DOI: 10.1002/edn3.70125

Y. O. Tynkevich, K. H. Blyzniuk, Y. I. Ivanovych, N. M. Roshka, A. I. Tokaryuk, A. Y. Shelyfist, R. A. Volkov (2025)
Genetic Diversity of Ukrainian Populations of Invasive Species of the Genus Galinsoga Assessed by ISSR-Markers. Cytology and Genetics 59: 11.

DOI: 10.3103/S0095452725010141

Mikihito Noguchi, Hirokazu Toju (2024)
Mycorrhizal and endophytic fungi structure forest below-ground symbiosis through contrasting but interdependent assembly processes. Environmental Microbiome 19: .

DOI: 10.1186/s40793-024-00628-8

K.R.L. Saranya, Jithu K. Jose, Praveen Kumar Sake, Siddharthan Surveswaran (2026)
DNA metabarcoding in forest ecosystems: current methods and future directions. Biodiversity 27: 292.

DOI: 10.1080/14888386.2026.2656989

Andrew Dyck, Roy V. Rea, Maria Kuzmina (2026)
Fecal DNA metabarcoding reveals spatial and temporal variation in the summer diets of moose (Alces alces) in north-central British Columbia. Forest Ecology and Management 605: 123498.

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