Primer Validation
Metabarcoding and Metagenomics 5: e68155
https://doi.org/10.3897/mbmg.5.68155 (02 Sep 2021)
https://doi.org/10.3897/mbmg.5.68155 (02 Sep 2021)
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Article title
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Abstract
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Key Words
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Introduction
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Material and methods
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De novo primer design
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In silico primer evaluation
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Mock community design
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Sequencing primer design
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PCR setup
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PCR optimisations
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Sequence data analysis
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Sequence data derived metrics
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Results
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In silico primer evaluation
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In silico primer evaluation of the SSU region
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In silico primer evaluation of the 5.8S nrDNA region (reverse)
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In silico primer evaluation of the 5.8S nrDNA region (forward)
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In silico primer evaluation of the LSU region
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Mock community sequence analysis
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Mock community primer tests
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Mock community PCR optimisations
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Discussion
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Selecting and refining: one possible approach to plant metabarcoding studies
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In silico primer evaluation
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Mock community primer test
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Mock community PCR optimisations
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Conclusion
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Funding statement
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Ethics statement
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Acknowledgements
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References
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Appendix 1
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Appendix 2
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Appendix 3
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Appendix 4
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Appendix 5
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Appendix 6
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Appendix 7
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Appendix 8
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