Methods
Metabarcoding and Metagenomics 5: e67533
https://doi.org/10.3897/mbmg.5.67533 (20 Jul 2021)
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  • Article title
  • Abstract
  • Key Words
  • Introduction
  • Materials and methods
    • Study design
    • Sample acquisition
    • Tissue homogenisation and cleaning
    • DNA extraction
    • qPCR validation, PCR, and library preparation
    • Bioinformatics, filtering, and statistical analysis
  • Results
    • Optical verification of homogenisation success
    • Amplification and melting curve analysis
    • Quantification of tag jumps
    • OTU richness and potential cross-contamination
    • Replicate consistency
  • Discussion
    • Cross-contamination
    • Replicate consistency
    • Proposition for routine application
  • Data accessibility statement
  • Acknowledgments
  • References
  • Supplementary materials
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