Population genetic haplotypic diversity assessed with metabarcoding assays versus traditional DNA sequencing (Experiment Series B). Round goby (RG) in Lake St. Clair (LSC2: surface, LSC3: benthos) and silver carp (SC) haplotypes in the Wabash River (WAB) assessed with traditional population genetic sequencing (Trad) and the GobyCytb and CarpCytb metabarcoding assays. New cytochrome b haplotypes (N) not described from either species to date, and having sequence frequencies <1% are unlabeled, for visual clarity.