A;Richness;Location;Morphology;Multi-assay;FishCytb;CarpCytb;GobyCytb;"12S RNA MiFish" "Maumee River 1";13;38;19;14;18;18 "Maumee River 2";22;25;15;12;9;7 "Maumee River 3";23;26;18;10;6;16 "Maumee River 4";23;28;17;6;15;19 "Maumee River 1–4";33;42;26;20;24;31 "Lake St. Clair 1";–;16;6;7;8;8 "Lake St. Clair 2";–;16;–;10;12;12 "Lake St. Clair 3";–;16;9;11;9;– "Lake St. Clair all";–;23;6;16;16;8 "Lake Erie Islands";–;14;7;8;–;9 "Wabash River 1";13;30;8;14;–;21 "Wabash River 2";12;29;14;10;5;16 "Wabash River 3";–;27;17;11;9;11 "Wabash River 1–2";18;37;22;20;10;30 B;Richness;"Unique to metabarcoding assays (false negatives)" Location;Morphology;Multi-assay;FishCytb;CarpCytb;GobyCytb;MiFish "Maumee River 1";13;"20 (0.00)";"1 (0.23)";"1 (0.38)";"3 (0.23)";"7 (0.31)" "Maumee River 2";22;"6 (0.41)";"3 (0.45)";"3 (0.73)";"1 (0.73)";"1 (0.77)" "Maumee River 3";23;"4 (0.26)";"0 (0.57)";"1 (0.70)";"0 (0.78)";"3 (0.52)" "Maumee River 4";23;"10 (0.30)";"1 (0.61)";"1 (0.83)";"4 (0.65)";"6 (0.57)" "Maumee River 1–4";33;"18 (0.12)";"2 (0.36)";"4 (0.67)";"6 (0.58)";"9 (0.39)" "Maumee River all";59;"9 (0.27)";"0 (0.56)";"2 (0.76)";"3 (0.64)";"6 (0.54)" "Wabash River 1";13;"16 (0.23)";"0 (0.54)";"5 (0.54)";–;"13 (0.46)" "Wabash River 2";12;"14 (0.08)";"7 (0.67)";"1 (0.50)";"2 (0.83)";"7 (0.17)" "Wabash River 1–2";18;"21 (0.00)";"9 (0.33)";"5 (0.39)";"2 (0.83)";"14 (0.22)"