Research Article
Metabarcoding and Metagenomics 4: e53455
https://doi.org/10.3897/mbmg.4.53455 (26 Aug 2020)
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  • ContentsContents
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  • Article title
  • Abstract
  • Key Words
  • Introduction
    • Potential error sources in environmental metabarcoding assays
    • Objectives
  • Methods
    • Ethics statement and fish sampling
    • Metabarcoding assays employed
    • Experiment Series A: Species compositions and community diversity
    • Experiment Series B: Population genetic variation with metabarcoding assays
    • DNA capture, extraction, and library preparation
    • Bioinformatic pipeline
    • Data analyses
  • Results
    • High-throughput sequencing metrics
    • Experiment Series A: Metabarcoding assays versus morphological identifications
    • Experiment Series A: Community comparisons
    • Experiment Series B: Intra-specific population diversity from metabarcoding assays
  • Discussion
    • Experiment Series A and B: Reducing error from metabarcoding assays
    • Experiment Series A: Relative abundances of species from metabarcoding assays
    • Experiment Series A: Resolution from metabarcoding assays versus conventional sampling
    • Experiment Series A: Community diversity patterns with metabarcoding assays
    • Experiment Series B: Population genetic patterns from metabarcoding results
  • Conclusion
  • Data resources
  • Acknowledgements
  • References
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