Total OTU (upper part) and haplotype number (lower part) summed across all seasons for all river systems (Em 1-6 – Emscher, En 1-7 – Ennepe 1-6, S – Sieg). A) All benthic macroinvertebrate taxa assigned to a reference sequence in BOLD with > 95% sequence identity; B) EPT taxa (Ephemeroptera, Plecoptera, Trichoptera); C) PR taxa (Pollution Resistant : Enchytraeida, Haplotaxida, Isopoda, Rhynchobdellida).

  Part of: Zizka VMA, Weiss M, Leese F (2020) Can metabarcoding resolve intraspecific genetic diversity changes to environmental stressors? A test case using river macrozoobenthos. Metabarcoding and Metagenomics 4: e51925.