Research Article
Metabarcoding and Metagenomics 4: e48281
https://doi.org/10.3897/mbmg.4.48281 (07 Feb 2020)
https://doi.org/10.3897/mbmg.4.48281 (07 Feb 2020)
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Article title
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Abstract
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Key Words
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Introduction
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Material and methods
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Reference genomes
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Selection of the species, preparation of the DNA libraries and sequencing
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Quality control and mapping
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From mapped reads to species identity
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Detection limit
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Selection of best values of γ, δ and ε
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Quantification of the relative proportion of the species
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Rarefaction of sequenced reads
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Statistics analysis
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Results
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Single-species libraries
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Mixed-species libraries
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Rarefaction of the reads
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Discussion
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Species identification: spurious species and the need for an analytical limit of detection
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Quantification of the relative abundance of the species
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Data treatment and the assignation of reads to species
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Present and future of metagenomics
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Concluding remarks
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Data accessibility
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Authors’ contributions
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Acknowledgements
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References
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Supplementary materials
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