Influence of sequencing depth on detected mutations and their effect sizes. Relationship between the number of investigated reads and number of mutations with significantly altered frequencies (A) and the average effect size for those mutations (B); as assessed using Mumame on shotgun metagenomic data from a ciprofloxacin exposure experiment.

  Part of: Magesh S, Jonsson V, Bengtsson-Palme J (2019) Mumame: a software tool for quantifying gene-specific point-mutations in shotgun metagenomic data. Metabarcoding and Metagenomics 3: e36236.