Primer Validation
Metabarcoding and Metagenomics 3: e33835
https://doi.org/10.3897/mbmg.3.33835 (24 Apr 2019)
https://doi.org/10.3897/mbmg.3.33835 (24 Apr 2019)
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Article title
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Abstract
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Key Words
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Introduction
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Materials and methods
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Primer development
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In silico evaluation of variation in the target region
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Test of primers with tissue extracted DNA
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Water sampling and filtration
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Library preparation and MiSeq sequencing with eDNA sample
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Data preprocessing and taxonomic assignment
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Results
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MiDeca primers
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In silico evaluation of variation in MiDeca sequence
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eDNA detection from natural seawater
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Discussion
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Usefulness of eDNA metabarcoding with MiDeca primers
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Taxa detected from eDNA metabarcoding
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Data accessibility
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Acknowledgements
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References