Observed DNA concentrations or number of Operational Taxonomic Units by experimental treatment. Observed DNA concentrations after DNA extraction (a) indexed PCR (PCR-2) (b) and the number of estimated OTUs per experimental treatment clustered at 97% for all eukaryotes and (c) and metazoans (d). The upper and lower whiskers indicate the minimum and maximum point within 1.5 times the Interquartile Range extended from the 25th and 75th percentile, respectively. Colours indicate filter material where red is Cellulose Nitrate (CN) and blue is Glass Fibre (GF). The three extraction methods are abbreviated as in Figure 1.

  Part of: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963