Methods
Metabarcoding and Metagenomics 2: e26869
https://doi.org/10.3897/mbmg.2.26869 (23 Aug 2018)
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  • ContentsContents
  • Article InfoArticle Info
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  • Article title
  • Abstract
  • Key Words
  • Introduction
  • Material and methods
    • Sampling procedure
    • Extraction of hyporheic meiofauna from sediment and DNA isolation
    • COI amplification and sequencing
    • Data quality filtering and MOTU clustering
    • Taxonomic assignment of MOTUs
    • Diversity analyses
  • Results
    • Taxonomic composition
    • Alpha diversity
    • Beta diversity
  • Discussion
    • Degenerate COI primers for metabarcoding of hyporheic communities
    • Site heterogeneity and metazoan community composition
    • The impact of replication strategy on inferred community composition
    • Future application scenarios
  • Conclusions
  • Data resources
  • Author contributions
  • Acknowledgements
  • References
  • Supplementary materials
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