Optimised Mu-DNA protocols for stool samples that previously failed to achieve inhibition-free DNA. Optimised protocols are compared to DNeasy PowerSoil. Total dsDNA yield, A260/A280, A260/A230 ratios and PCR indices are shown for stool samples B and C. Horizontal dashed lines indicate ideal measures of A260/A280 and A260/A230 ratios for pure DNA. Asterisks indicate significant differences between methods (planned contrast linear model, p<0.05).

 
 
  Part of: Sellers GS, Di Muri C, Gómez A, Hänfling B (2018) Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types. Metabarcoding and Metagenomics 2: e24556. https://doi.org/10.3897/mbmg.2.24556