Research Article
Metabarcoding and Metagenomics 2: e23297
https://doi.org/10.3897/mbmg.2.23297 (14 Mar 2018)
https://doi.org/10.3897/mbmg.2.23297 (14 Mar 2018)
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Introduction
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Material and methods
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Study site
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Water sampling and DNA extraction
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Preparation of standard fish DNAs
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Paired-end library preparation
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Sequence read processing and taxonomic assignment
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Determination of the number of eDNA copies by quantitative PCR
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Statistical analysis
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Data and code availability
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Results and discussion
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Relationship between the copy numbers and sequence reads of the standard DNA
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Quantification of the copy number using sequence reads and correction equations and comparison of the calculated copy number with the copy number quantified by qPCR
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Temporal dynamics of Maizuru-bay fish eDNA revealed by qMiSeq
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Quantitative and multispecies fish eDNA monitoring
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Conclusions
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Acknowledgements
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References
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Supplementary files
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