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  • ContentsContents
  • Article InfoArticle Info
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  • Inst. CodeInst. Code
  • Article title
  • Abstract
  • Key words
  • Introduction
  • Methods
    • Tissue samples and mitochondrial genome sequencing
    • Assay design and validation testing
    • Limit of Detection (LOD) and Limit of Quantification (LOQ)
    • Specificity and inhibition testing
    • Assay validation: environmental DNA samples
  • Results
    • Mitochondrial genomes
    • Assay design, optimisation, and validation
    • Assay LOD and LOQ
    • Empirical LOQ
    • Environment-based inhibition
    • Environmental DNA samples
  • Discussion
    • Challenges in monitoring subterranean fauna
    • Assay performance and implications
    • Genetic insights and future directions
  • Acknowledgements
  • References
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Artificial Intelligence (AI) use
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • Supplementary material
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