Other versions:

(Verbatim name)

ID

(Catalog Number)

ORG

DES

Invalid DOI was provided, please try again

XML
PDF
 
 
Bluesky
Twitter
Facebook
Telegram
Mendeley
Reddit
Notify a colleague
  • ContentsContents
  • Article InfoArticle Info
  • CiteCite
  • MetricsMetrics
  • CommentComment
  • RelatedRelated
  • FigsFigs
  • TabsTabs
  • TaxaTaxa
  • DataData
  • RefsRefs
  • CitedCited
  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Study sites and eDNA collection
    • Species-specific primer set development for Nanopore sequencing
    • PCR and circularisation of PCR products for long-read amplicon sequencing
    • Bioinformatics analysis
    • Haplotype identification and sequence pair concatenation
    • Statistical analyses
    • Phylogenetic analyses
    • Divergence time estimation
    • Library preparation for quantitative fish eDNA metabarcoding
  • Results
    • Sequencing of 1,000 bp from eDNA samples and haplotyping
    • Phylogenetic relationships and phylogeographic patterns
    • Divergence times inferred from concatenated sequences
    • Quantitative fish eDNA metabarcoding
  • Discussion
    • Requirements and practical feasibility of long-fragment eDNA sequencing
    • A practical balance of resolution, labour, and financial cost
    • High-resolution phylogeographic structure of B. oreas
    • Broader applicability and limitations of long-fragment eDNA sequencing
  • Acknowledgements
  • References
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Artificial Intelligence (AI) use
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • Supplementary materials
Subscribe to email alerts for current Article's categories
Powered by