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  • Article InfoArticle Info
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • China common invasive species list and mPCR primers design
    • Invasive species standard plasmid design
    • Mix plasmid amplification
    • Pearl river environmental DNA collection and extraction
    • mPCR and metabarcoding with environmental samples
    • High-throughput sequencing and bioinformatics analysis
  • Results
    • Validation of mPCR amplification using mixed standard plasmids
    • Invasive species monitoring in the Pearl River Estuary using mPCR
    • Comparison of metabarcoding and mPCR
  • Discussion
    • Accuracy of mPCR sequencing
    • Consistency between mPCR and metabarcoding
    • Ecological impacts and prevention of invasive species
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Use of AI
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary material
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