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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Study location
    • Identification of Ae. aegypti larvae in the field and collection of samples
    • Temperature and pH of water in the breeding sites
    • Isolation of microalgae from a water sample
    • Dissection of larval gut and isolation of microalgae from gut contents of Ae. aegypti larvae
    • Morphological identification of microalgae
    • Genomic DNA extraction of microalgae
    • Genetic marker amplification and bioinformatics
    • Bioinformatics
    • Alpha diversity of microalgal community
    • Beta diversity of microalgal community
  • Results
    • Temperature and pH of water in the breeding site
    • Morphological identification of microalgae
    • Metabarcoding using 23S rDNA amplicon sequencing of microalgal communities
    • Alpha diversity
    • Beta diversity
  • Discussion
  • Conclusions
  • Acknowledgements
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Use of AI
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary materials
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