Primer Validation
Metabarcoding and Metagenomics 1: e14625
https://doi.org/10.3897/mbmg.1.14625 (20 Sep 2017)
https://doi.org/10.3897/mbmg.1.14625 (20 Sep 2017)
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Article metadata
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Introduction
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Material and Methods
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Primer development
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In silico evaluation of primers
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Sample collection and processing
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DNA extraction and tissue pooling
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DNA metabarcoding and bioinformatics
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Results
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Primer design and in silico evaluation
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Metabarcoding and Illumina sequencing
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Taxa recovery in mock and bulk samples
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Discussion
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Primer development and performance
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Validation of sequence / biomass relationships within species
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Conclusions
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Acknowledgements
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References
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Supplementary files
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