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  • ContentsContents
  • Article InfoArticle Info
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  • CommentComment
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  • FigsFigs
  • TaxaTaxa
  • DataData
  • RefsRefs
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Study site
    • Macroinvertebrate sampling
    • Laboratory procedures
      • Morphological classification
      • Tissue homogenization
      • DNA extraction and quantification
      • PCR amplification
      • Library prep/cleanup/normalization
      • Sequencing
    • Data analysis
      • Sequence Processing
      • Taxonomic designation
      • Biotic index calculation
  • Results
    • Molecular-based identification
    • Morphological-based identification
    • Comparison between methodologies
    • Biological index
  • Discussion
  • Conclusion
  • Acknowledgments
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary material
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