Organisation of the cross-laboratory experiments

Collection and preparation of calibrated samples

Proficiency tests

Methods comparison experiments

Library preparation and HTS sequencing

Sequencing data processing

Morphological data

Data analysis of proficiency tests (E1 and E2)

Data analysis of method comparison experiments (E3 and E4)

Feedbacks from experiments

Key molecular and morphological working tables

Proficiency tests (E1 and E2)

Methods comparison experiments (E3 and E4)

IBD and IPS morphological scores

Validation of the proficiency tests shows that the applied DNA and PCR protocols can perform reproducible molecular diatom freshwater biomonitoring

“All for one, but prove them all”: establishing proficiency testing schemes for performance-based quality management of DNA-based biomonitoring

Intercalibration and inter-comparison exercises as tools for integration of DNA approach along morphology for freshwater routine monitoring

Conflict of interest

Ethical statement

Funding

Author contributions

Author ORCIDs

Data availability