Increased species-level identification accuracy for the 90 samples processed using the workflow described in this work. The 90 samples were processed using MiSeq metabarcoding and a publicly available database. This highlighted a number of incorrect taxonomical assignments (in red). The morphological examination and resequencing of COI fragments from the voucher specimens preserved post non-destructive DNA extraction enabled provision of species-level identification for most of the samples (in green) and to use these curated sequences for the taxonomical assignment of the next metabarcoding analysis conducted using MinION. Parts of this figure were created with BioRender.com.