Volcano plot showing the fold change in abundance of the genus and species between the original and RA sample analyses. The horizontal dotted line denotes significance (p <0.05), and the vertical lines denote the magnitude of difference, where the lines are set at the equivalent of a 4-fold difference. Points to the right denote taxa found more abundantly in the original analysis, and points to the left denote taxa more abundant in the repeat analysis. Similar pairwise comparisons were made between all three groups (OG, RA, and RB), which also showed no significant difference (see Suppl. material 3).