Nematode Presence/Absence Detection Method Comparison Heatmap. Binary heatmap of detected taxa and trophic groups, based on morphology, cox1 read-mapping, all mitochondrial genes read-mapping and annotated COX1 (black = detected, white = not detected). Trophic groups detected in fields included Bacterial Feeders (BF; blue), Plant Parasites (PP; Green), Fungal Feeders (FF; brown), Animal parasites (AP; red), Entomopathogenic Nematodes (EPN; yellow), Predators (PR; black) and Omnivores (OM; grey). Detections are split by field of origin, site sampled, site replicates and depth. Annotated COX1 detections were the most similar to the morphology data of any mtMG method and so only the detected species for the Annotated COX1 data are presented.