Research Article
Metabarcoding and Metagenomics 8: e121817
https://doi.org/10.3897/mbmg.8.121817 (29 May 2024)
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Material and methods
    • Sample collection
    • DNA extraction and PCR amplifications
    • Library constructions and sequencing
    • Sequence reads processing
    • Taxonomic assignments
    • Statistics
  • Results
    • Overview of sequence data
    • Macrofaunal-based biotic index of LIS samples
    • Shannon diversity
    • Beta-diversity
    • Taxonomic assignments
  • Discussion
    • Data quality of Nanopore duplex reads
    • Comparison of ecological trends inferred from Nanopore- and Illumina-derived bacterial diversity patterns
    • Low taxonomic resolution of short Illumina reads affects taxonomy-based bacterial diversity patterns
    • PCR primer bias and stochasticity may affect analyses of bacterial community structures obtained from Nanopore and Illumina sequencing
  • Conclusions
  • Acknowledgements
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary material
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