Research Article
Metabarcoding and Metagenomics 8: e115512
https://doi.org/10.3897/mbmg.8.115512 (20 Aug 2024)
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Study area and sampling design
    • Sampling: eDNA and traditional field survey
    • Metabarcoding of vertebrate species
    • Quantitative PCR of target species L. lutra
      • Primer validation
      • Detection thresholds and quantification
  • Results
    • Field survey results based on observation and literature review
    • Comparing the detectability Eurasian Otters using eDNA: Metabarcoding vs. qPCR
    • The evaluation of distribution pattern and food species estimation
      • Distribution patterns of L. lutra by seasons
    • The estimation of potential food species for L. lutra in Saetgang
  • Discussion
    • The ecological characteristics of Eurasian otters in the Yeouido Ecological Park
    • How to improve the detection probability and reliability of rare species
    • Limitations and potentials for habitat evaluation and distribution patterns of semi-aquatic mammals with eDNA survey
  • Conclusions
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary materials
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