Schematic representation of the Natrix2 workflow. The processing of two split samples using AmpliconDuo is depicted. The color scheme represents the main steps, dashed lines outline the OTU and dotted edges outline the ASV variant of the workflow. Stars depict updates to the original Natrix workflow. Details on the ONT part are depicted in Fig. 2. (Created with BioRender.com).

 
 
  Part of: Deep A, Bludau D, Welzel M, Clemens S, Heider D, Boenigk J, Beisser D (2023) Natrix2 – Improved amplicon workflow with novel Oxford Nanopore Technologies support and enhancements in clustering, classification and taxonomic databases. Metabarcoding and Metagenomics 7: e109389. https://doi.org/10.3897/mbmg.7.109389