Research Article
Metabarcoding and Metagenomics 8: e104655
https://doi.org/10.3897/mbmg.8.104655 (24 Jul 2024)
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Methods
    • Study lakes and water sample collection
    • Water filtration and DNA extraction
    • Sequencing library preparation
    • Bioinformatics and data set clean-up
    • Biodiversity metrics
    • Effect of sample number on lake fish biodiversity metrics
      • Species accumulation curves and statistical estimation of sampling threshold
      • Random resampling of lake fish eDNA metabarcoding data
      • Non-random reduced sampling of lake metabarcoding data
    • Shoreline sampling validation
  • Results
    • Bioinformatics and data set clean-up
    • Effect of sample number on lake fish species biodiversity metrics
      • Species accumulation curves
      • Sampling threshold
      • Random resampling of lake metabarcoding data
      • Non-random reduced sampling of lake fish species metabarcoding data
    • Shoreline sampling validation
  • Discussion
    • Effect of reduced sampling on species detection and community composition estimation
    • Spatio-temporal considerations of sampling
  • Conclusion
  • Acknowledgments
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary material
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