Proportions of fish and non-fish OTUs (A) and read proportions (B) detected with the five different primer pairs (A), and the proportions of ambiguous taxonomic assignments (flags 1–4) for all species-level OTUs (C), based on the pre-adjusted datasets.

  Part of: Macher T-H, Schütz R, Yildiz A, Beermann AJ, Leese F (2023) Evaluating five primer pairs for environmental DNA metabarcoding of Central European fish species based on mock communities. Metabarcoding and Metagenomics 7: e103856.