Individual read abundances resulting from varying amounts of purified total DNA or target DNA fragments. Read abundance of specimens in artificial communities when using different amounts of purified DNA (orange colours represent four mock communities) or different amounts of target DNA fragments (grey, four mock communities) per specimen for community pools. DNA input units correspond to 0.05 ng of purified total DNA and 1 ng of target DNA fragments. Shown are regression lines and multiple R2 of the fitted linear regression models.

 
 
  Part of: Röder N, Schwenk K (2023) Direct PCR meets high-throughput sequencing – metabarcoding of chironomid communities without DNA extraction. Metabarcoding and Metagenomics 7: e102455. https://doi.org/10.3897/mbmg.7.102455